Journal: Cells

Article Title: Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity

doi: 10.3390/cells12192342

Figure Lengend Snippet: CAP3/St14 knockout mice showed altered protein abundancies for ENaC subunits, NCC, and NKCC2 and downregulation of CAP1/Prss8 upon Na + deprivation. ( A ) Representative Western blot analyses of CAP3, CAP1, αENaC, αENaC CL (cleaved), βENaC, γENaC, γENaC CL (cleaved), NCC, pNCC, NHE3, NKCC2, ROMK and BK on kidney lysates from controls (black circles, n = 6) and CAP3/St14 knockout (CAP3/St14 Pax8LC1 , white circles, n = 6). Kidney lysates of control and renal tubular-specific knockouts of αENaC, βENaC, and γENaC served as negative (-ctl) and positive (+ctl) controls, respectively. ( B ) Quantification of data. Results are presented as mean ± SEM. Data were analyzed using an unpaired two-tailed Welch’s t -test, and p values <0.05 were considered statistically significant; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The following primary antibodies were used with dilution 1/1000: αENaC, βENaC, NCC, pNCC (T53), NHE3, NKCC2 [ , ], γENaC (StressMarq, Biosciences, Victoria, Canasa) [ , ], CAP3/St14 (R&D systems AF3946, Minneapolis, MN, USA), BK/Maxi-Ki, Kca1.1 (APC-151, Alomone labs, Jerusalem, Israel).

Techniques: Knock-Out, Western Blot, Control, Two Tailed Test

Journal: Cells

Article Title: Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity

doi: 10.3390/cells12192342

Figure Lengend Snippet: CAP1/Prss8; CAP3/St14 double knockout mice showed normalized ENaC subunit but still lowered pNCC and BK protein abundances under a low Na + diet. ( A ) Representative Western blot analysis of CAP3, CAP1, αENaC, αENaC CL (cleaved), βENaC, γENaC, γENaC CL (cleaved), NCC, pNCC, NHE3, NKCC2, ROMK, and BK on kidney lysates from controls (black squares, n = 5) and CAP1/Prss8; CAP3/St14 double knockout (CAP1/Prss8; CAP3/St14 Pax8LC1 , n = 7) mice. Kidney lysates of control and renal tubular-specific knockouts of αENaC, βENaC, and γENaC served as negative (−ctl) and positive (+ctl) controls, respectively. ( B ) Quantification of the data. Results are presented as mean ± SEM. Data were analyzed using an unpaired two-tailed Welch’s t -test. p values < 0.05 were considered statistically significant; * p < 0.05, *** p < 0.001.

Article Snippet: The following primary antibodies were used with dilution 1/1000: αENaC, βENaC, NCC, pNCC (T53), NHE3, NKCC2 [ , ], γENaC (StressMarq, Biosciences, Victoria, Canasa) [ , ], CAP3/St14 (R&D systems AF3946, Minneapolis, MN, USA), BK/Maxi-Ki, Kca1.1 (APC-151, Alomone labs, Jerusalem, Israel).

Techniques: Double Knockout, Western Blot, Control, Two Tailed Test

Journal: Kidney international

Article Title: Renal tubular dysfunction in patients with American cutaneous leishmaniasis.

doi: 10.1038/ki.2011.251

Figure Lengend Snippet: Figure 2 | Western blotting analysis in the urinary exosome fraction, normalized to urinary creatinine, in controls and in patients with ACL. Abundance of (a) AQP2 and (b) NKCC2. ACL, American cutaneous leishmaniasis; AQP2, aquaporin 2; NKCC2, Na-K-2Cl cotransporter.

Article Snippet: We obtained the peptide-derived polyclonal antibodies specific to AQP2, NKCC2, NHE3, and pendrin from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Western Blot

Journal: Cell reports

Article Title: Magnesium and Calcium Homeostasis Depend on KCTD1 Function in the Distal Nephron

doi: 10.1016/j.celrep.2020.108616

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: rabbit anti-NKCC2 , Cell Signaling Technologies , Cat# 38436, RRID:AB_2799134.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Plasmid Preparation